Primary structure of bovine collectin-43 (CL-43). Comparison with conglutinin and lung surfactant protein-D.
نویسندگان
چکیده
منابع مشابه
Conglutinin and CL-43, two collagenous C-type lectins (collectins) in bovine serum.
Introduction Hefore the turn of the century proteins in plant seed extracts were found to agglutinate erythrocytes. Such proteins were used extensively in early investigations of the immune system, notably by Paul Erlich. Half a century later, it was found that the agglutination could be inhibited by simple sugars and the proteins responsible were named lectins. The first similar mammalian aggl...
متن کاملPurification and characterization of a bovine serum lectin (CL-43) with structural homology to conglutinin and SP-D and carbohydrate specificity similar to mannan-binding protein.
A previously undescribed bovine serum lectin (designated CL-43) was identified by its Ca(2+)-dependent binding to mannan and by its molecular mass of 43 kDa under reducing conditions on SDS-PAGE. The lectin was isolated by polyethylene glycol precipitation, affinity chromatography on mannan-Sepharose (followed by elution with EDTA), and absorption on Sepharose-4B-coupled rabbit anti-bovine Ig (...
متن کاملAssignment of the surfactant protein A gene (SFTPA) to bovine chromosome 28q1.8-->q1.9 by radiation hybrid mapping.
The collectin surfactant protein A (SP-A) is involved in the innate host defense of the lung. It binds to non-self glyco-conjugates on the surface of invading microorganisms. Binding leads to lysis, aggregation and initiation of effector mechanisms that include increased phagocytosis by phagocytic cells and modulation of the inflammatory response (Haagsman, 2002). Across species, the gene encod...
متن کاملStructural characterization of human and bovine lung surfactant protein D.
Human and bovine surfactant proteins D (SP-D) were purified from late amniotic fluid and bronchioalveolar lavage on the basis of its Ca(2+)-dependent affinity for maltose. The molecular mass of a trimeric subunit was determined by matrix-assisted laser desorption ionization MS to lie in the range 115-125 kDa for human SP-D and 110-123 kDa for bovine SP-D. A single polypeptide chain was determin...
متن کاملCL-46, a novel collectin highly expressed in bovine thymus and liver.
Collectins are oligomeric molecules with C-type lectin domains attached to collagen-like regions via alpha-helical neck regions. They bind nonself glycoconjugates on the surface of microorganisms and inhibit infection by direct neutralization, agglutination, or opsonization. During the characterization of the gene encoding bovine CL-43 (43-kDa collectin), we identified a novel collectin-gene. W...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1994
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)32646-7